serum-free imdm Search Results


serum-free imdm  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc serum-free imdm
Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs <t>were</t> <t>cultured</t> in <t>IMDM</t> containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.
Serum Free Imdm, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+imdm/serum+free+imdm/pmc06036281-46-24-26
Average 90 stars, based on 1 article reviews
serum-free imdm - by Bioz Stars, 2026-11
90/100 stars
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serum-free imdm  (EuroClone)
90
EuroClone serum-free imdm
Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs <t>were</t> <t>cultured</t> in <t>IMDM</t> containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.
Serum Free Imdm, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+imdm/serum+free+imdm/us08268619-86-8-10
Average 90 stars, based on 1 article reviews
serum-free imdm - by Bioz Stars, 2026-11
90/100 stars
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serum-free imdm  (Avantor)
90
Avantor serum-free imdm
Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs <t>were</t> <t>cultured</t> in <t>IMDM</t> containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.
Serum Free Imdm, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+imdm/serum+free+imdm/pmc09240779-65-10-12
Average 90 stars, based on 1 article reviews
serum-free imdm - by Bioz Stars, 2026-11
90/100 stars
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semisolid serum-free imdm culture medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc semisolid serum-free imdm culture medium
Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs <t>were</t> <t>cultured</t> in <t>IMDM</t> containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.
Semisolid Serum Free Imdm Culture Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+imdm/semisolid+serum+free+imdm+culture+medium/pm11154221-62-15-23
Average 90 stars, based on 1 article reviews
semisolid serum-free imdm culture medium - by Bioz Stars, 2026-11
90/100 stars
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serum free imdm  (Cellgro)
86
Cellgro serum free imdm
Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs <t>were</t> <t>cultured</t> in <t>IMDM</t> containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.
Serum Free Imdm, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+imdm/free+imdm+serum/us12655222-1125-17-19
Average 86 stars, based on 1 article reviews
serum free imdm - by Bioz Stars, 2026-11
86/100 stars
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Image Search Results


Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.

Journal: Frontiers in Pharmacology

Article Title: The IGF2/IGF1R/Nanog Signaling Pathway Regulates the Proliferation of Acute Myeloid Leukemia Stem Cells

doi: 10.3389/fphar.2018.00687

Figure Lengend Snippet: Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition ( ∗ P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.

Article Snippet: LSCs from KG-1a and MOLM13 were isolated using a magnetic-activated cell-sorting (MACS) kit (Cat No. 130-056-701; Miltenyi Biotec, Bergisch Gladbach, Germany) and cultured in serum-free IMDM (STEMCELL Technologies, Vancouver, BC, Canada) containing 20 ng/mL basic fibroblast growth factor (bFGF; PeproTech, Rocky Hill, NJ, United States), 20 ng/mL epidermal growth factor (EGF; PeproTech), and B27 media (1:50; Life Technologies, Carlsbad, CA, United States).

Techniques: Expressing, Cell Culture, Incubation, Western Blot